Product Description

SR-4 (D47) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2390-50, BS2390-100

Sizes: 50µl, 100µl

Swiss-Prot: Q13639

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: Serotonin (also designated 5-hydroxytryptamine or 5-HT) is a molecule that functions as a neurotransmitter, a hormone and a mitogen, and it is predominantly expressed in the gut, platelets and central nervous system (CNS). In the CNS, serotonin modulates several processes, including anxiety, sleep, appetite, behavior and drug abuse. In platelets and gut, serotonin plays a major role in cardiovascular function and motility of the gastrointestinal tract, respectively. Serotonin mediates its effects through several of G protein coupled receptors, designated 5-HT receptors or alternatively SR receptors. SR-3 is a ligand-gated ion channel, whereas all other known serotonin receptor subtypes are G protein-coupled receptors. The gene which encodes SR-3 maps to human chromosome 11q23.1-q23.2. SR-4 mediates widespread effects in central and peripheral nervous systems. The gene which encodes SR-4 maps to human chromosome 5q31-q33. SR-7 belongs to the superfamily of G protein-coupled receptors.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: SR-4 (D47) polyclonal antibody detects endogenous levels of SR-4 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SR-4 (D47) pAb at 1:500 dilution
Lane 1: CT26 whole cell lysate (40ug)
Lane 2: C6 whole cell lysate (40ug)
Lane 3: HEK293T whole cell lysate (40ug)
Lane 4: K562 whole cell lysate (40ug)
Lane 5: U-87MG whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of SR-4 (D47) pAb in paraffin-embedded human brain tissue.

Bioworld Molecular Weight: ~ 48 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: 5-hydroxytryptamine receptor 4; 5-HT-4; 5-HT4; Serotonin receptor 4; HTR4

Immunogen: Synthetic peptide, corresponding to amino acids 14-66 of Human SR-4.

Conjugate: Unconjugated

Modification: Unmodified