SRA1 Polyclonal Antibody-BS60560

SRA1 Polyclonal Antibody

Catalogue Numbers: BS60560-50, BS60560-100

Sizes: 50µl, 100µl

Alternative Name: Steroid receptor RNA activator 1; Steroid receptor RNA activator protein; SRAP; SRA1; PP7684

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9HD15

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Steroid receptor RNA activator (SRA) selectively mediates transactivation of steroid hormone receptors. Specifically, SRA exists as both an RNA transcript that forms a complex with steroid receptor coactivator-1 and as a stably expressed protein. There are six RNA motifs in SRA that are important for coactivation. SRA is ubiquitously expressed in normal tissues with higher levels of expression in liver and skeletal muscle. SRA is expressed at a low level in brain. SRA is expressed at higher levels in breast tumor than in normal tissue. Overexpression of SRA stimulates ERalpha transcriptional activity. In cells transfected with antisense oligodeoxynucleotides to SRA, ERalpha expression is reduced in a dose-dependent fashion. SMRT/HDAC1 associated repressor protein (SHARP) binds to SRA and inhibits SRA-potentiated steroid receptor transciption.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SRA1 polyclonal antibody detects endogenous levels of SRA1 protein.

Bioworld Molecular Weight: ~ 26 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SRA1 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human SRA1.

Conjugate: Unconjugated

Modification: Unmodified