Product Description

Src Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11632-50, MB11632-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P12931

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100

Background: Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 60 kDa; Observed MW: 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Src in SH-SY5Y, U87-MG lysates using Src antibody., Western blot analysis of Src in C6, 3T3 lysates using Src antibody., Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Src antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src

Immunogen: A synthetic peptide of human Src

Conjugate: Unconjugated

Modification: Unmodified