Product Description

SRD5A1 Polyclonal Antibody

Catalogue Numbers: BS60411-50, BS60411-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P18405

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:500~1:1000

Background: In cultured human skin cells, 5a-Reductase 1 shows heterogeneity of protein, and has different levels of transcriptional and translational expression. 5a-Reductase 1 is expressed in all portions of the hair follicle, whereas 5a-Reductase 2 is expressed only in mesenchymal portions. In addition, 5a-Reductase 1 is mainly expressed in human breast carcinoma and may play a role in the in situ production and actions of the potent androgen 5a-dihydrotestosterone, including inhibition of cancer cell proliferation in hormone-dependent human breast carcinoma. The 5a-Reductase-3a-hydroxysteroid dehydrogenase complex is present in the human brain, suggesting that the complex may be involved in the synthesis of neuroactive steroids or the catabolism of neurotoxic steroids.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SRD5A1 polyclonal antibody detects endogenous levels of SRD5A1 protein.

Extra Notes: Western blot (WB) analysis of SRD5A1 pAb at 1:500 dilution
Lane1:HEK293T whole cell lysate(40ug)
Lane2:PC3 whole cell lysate(40ug)
Lane1:A549 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 28 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: 3-oxo-5-alpha-steroid 4-dehydrogenase 1; SR type 1; Steroid 5-alpha-reductase 1; S5AR 1

Immunogen: A synthetic peptide corresponding to residues in Human SRD5A1.

Conjugate: Unconjugated

Modification: Unmodified