Product Description

SREBP-1 (phospho Ser439)Polyclonal Antibody

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65383-50, BS65383-100

Swiss-Prot: P36956

Host: Rabbit

SREBP-1 (phospho Ser439)Polyclonal Antibody-BS65383

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Rat

Applications: WB, IHC-P, IF (paraffin section), ELISA

All Applications: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Background: sterol regulatory element binding transcription factor 1(SREBF1) Homo sapiens This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Mar 2016],

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity: Phospho-SREBP-1 (S439) Polyclonal Antibody detects endogenous levels of SREBP-1 protein only when phosphorylated at S439.

Bioworld Molecular Weight: ~122 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry analysis of paraffin-embedded human brain, using SREBP-1 (Phospho-Ser439) Antibody. The picture on the right is blocked with the phospho peptide., Western blot analysis ofLysates from Jurkat cells treated with TNF 20ng/ml 30', using SREBP-1 (Phospho-Ser439) Antibody. The lane on the right is blocked with the phospho peptide.

Alternative Name: SREBF1; BHLHD1; SREBP1; Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1

Immunogen: The antiserum was produced against synthesized peptide derived from human SREBP-1 around the phosphorylation site of Ser439. AA range:405-454

Modification: Phosphorylation

Conjugate: Unconjugated