SREBP-1c Polyclonal Antibody Sizes: 50μl, 100μl Catalogue Numbers: BS67746-50, BS67746-100 Lead times: 1-2 weeks, if manufacturer has product in stock Manufacturer/Ship Location: China Background: The low density lipoprotein (LDL) receptor mediates the endocytic uptake of cholesterol-carrying lipoproteins, thereby controlling cholesterol levels in cells and plasma. Transcription of the LDL receptor gene is controlled by a ten base pair sequence in the 5' flanking region, designated sterol regμlatory element 1 (SRE-1). When cellμlar sterol stores are depleted, the element is activated, the gene is transcribed and the cellμlar uptake of LDL increases. A set of SREbinding proteins (SREBPs) have been identified, including two basic helixloop-helix leuicine zipper (bHLH-Zip) transcription factors, designated SREBP-1 and SREBP-2. SREBP-1 (also designated ADD1, for adipocyte determination and differentiation factor) is synthesized as a precursor that is attached to the nuclear envelope and endoplasmic reticμlum. In sterol-depleted cells, the membrane-bound precursor is cleaved to generate a soluble NH2-terminal fragment that translocates to the nucleus to activate transcription. Sterols inhibit the cleavage of SREBP-1. Category: Primary Antibody Reactivity: Human, Mouse, Rat Host: Rabbit Applications: WB, IHC Alternate Names: BHLHD1, SREBP1, Sterol regμlatory element-binding protein 1, SREBP-1, Class D basic helix-loop-helix protein 1, bHLHd1, Sterol regμlatory element-binding transcription factor 1 Clonality: Polyclonal Conjugate: Unconjugated Modification: Unmodification Immunogen: Recombinant protein corresponding to human SREBP-1c. The exact sequence is proprietary. Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/200) Purification: The antibody was purified by immunogen affinity chromatography. Specificity: Recognizes endogenous levels of SREBP-1c protein. Molecular Weight: ~ 72 kDa Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. SwissProt: P36956 Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Research Use Only