Product Description

SRF (N153) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS9121-50, BS9121-100

Sizes: 50µl, 100µl

Swiss-Prot: P11831

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC

Background: Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk-1 and SRF accessory protein-1 (SAP-1) have DNA binding properties identical to that of p62TCF. Elk-1 and SAP-1 contain two homologous regions of which the two amino-terminal regions, the Ets domain (box A) and the B box, mediate ternary complex formation with SRF.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: SRF (N153) polyclonal antibody detects endogenous levels of SRF protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SRF (N153) polyclonal antibody at 1:500 dillution
Lane 1: HEK293T whole cell lysate (40μg)
Lane 2: 786-O whole cell lysate (40μg)
Lane 3: CT-26 whole cell lysate (40μg), Immunohistochemistry (IHC) analyzes of SRF (N153) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~67 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Serum response factor

Immunogen: Synthetic peptide, corresponding to amino acids 121-170 of Human SRF.

Conjugate: Unconjugated

Modification: Unmodified