Product Description

SRF (phospho-S103) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4177-50, BS4177-100

Swiss-Prot: P11831

Host: Rabbit

SRF (phospho-S103) Polyclonal Antibody-BS4177

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk-1 and SRF accessory protein-1 (SAP-1) have DNA binding properties identical to that of p62TCF. Elk-1 and SAP-1 contain two homologous regions of which the two amino-terminal regions, the Ets domain (box A) and the B box, mediate ternary complex formation with SRF.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-SRF (S103) polyclonal antibody detects endogenous levels of SRF protein when phosphorylated at Ser103.

Bioworld Molecular Weight: ~ 52 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SRF (phospho-S103) polyclonal antibody at 1:500 dilution
Lane 1: H1792 whole cell lysate (40ug)
Lane 2: SK-OVCAR3 whole cell lysate (40ug)
Lane 3: HEK293T whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-SRF (S103) pAb in paraffin-embedded human lung cancer tissue.

Alternative Name: Serum response factor

Immunogen: Synthetic phosphopeptide derived from human SRF around the phosphorylation site of Serine 103.

Modification: Phosphorylation

Conjugate: Unconjugated