SRp46 (E72) Polyclonal Antibody-BS9165

SRp46 (E72) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS9165-50, BS9165-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9BRL6

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/serine (RS)-rich domain, are essential for constitutive splicing and also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites. The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport. The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing-sites. An additional SR-associated protein, p32, tightly associates with SR factors and preferentially inhibits ASF/SF2 functioning as both a splicing enhancer and splicing repressor protein by preventing the stable interaction of ASF/SF2 and the RNA.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: SRp46 (E72) polyclonal antibody detects endogenous levels of SRp46 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SRp46 (E72) pAb at 1:500 dilution
Lane 1: PC12 whole cell lysate (40ug)
Lane 2: The Embryo tissue lysate of Mouse (40ug)
Lane 3: EC9706 whole cell lysate (20ug)
Lane 4: HEK293T whole cell lysate (10ug)
Lane 5: A549 whole cell lysate (20ug), Immunohistochemistry (IHC) analyzes of SRp46 (E72) pAb in paraffin-embedded human colon cancer tissue.

Bioworld Molecular Weight: ~ 35 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: arginine/serine-rich 2B; Pre mRNA splicing factor SRP46; Pre-mRNA-splicing factor SRP46; Serine/arginine-rich splicing factor 8; SFRS2B; Splicing factor; Splicing factor arginine serine rich 46kD; Splicing factor arginine serine rich 2B; Splicing factor SRp46; SRP46; SRSF8; SRSF8;

Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human SRp46.

Conjugate: Unconjugated

Modification: Unmodified