SRp75 (D134) Polyclonal Antibody-BS2750

SRp75 (D134) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2750-50, BS2750-100

Sizes: 50µl, 100µl

Swiss-Prot: Q08170

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Serine/arginine-rich protein 75 (SRp75) is encoded by the SRSF4 gene. SRp75 belongs to the arginine/serine-rich factor family and likely plays a role in alternative mRNA splicing.The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/ serine (RS)-rich domain. They are not only essential for constitutive splicing but also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites. The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport. The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing sites.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: SRp75 (D134) polyclonal antibody detects endogenous levels of SRp75 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of SRp75 pAb at 1:500 dilution
Lane 1: HepG2 whole cell lysate (40ug)
Lane 2: H1792 whole cell lysate (40ug)
Lane 3: PC12 whole cell lysate (40ug)
Lane 4: AML-12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of SRp75 (D134) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 57 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: arginine/serine-rich 4; Pre mRNA splicing factor SRP75; Pre-mRNA-splicing factor SRP75; Serine/arginine-rich splicing factor 4; Splicing factor; Splicing factor; arginine/serine rich 4; SRP001LB; SRP75; Srsf4;

Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human SRp75.

Conjugate: Unconjugated

Modification: Unmodified