Product Description

SSCA1/SerpinB3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7581-50, BS7581-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P29508

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: Serpin B3, also known as squamous cell carcinoma antigen-1 (SCCA-1), is a member of the serpin superfamily of serine protease inhibitors. Serpin B3 belongs to the subgroup ovalbumin-related serpins which are involved in the regulation of apoptosis, inflammation, angiogenesis and embryogenesis. It may act as a papain-like cysteine protease inhibitor to modulate the host immune response against tumor cells. It also functions as an inhibitor of UV-induced apoptosis via suppression of the activity of c-Jun NH(2)-terminal kinase (JNK1).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 45KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of 293T, using SSCA1/SerpinB3 antibody at 1:1020 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunofluorescence analysis of A431 cells using SSCA1/SerpinB3 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of HepG2 cells using SSCA1/SerpinB3 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: SERPINB3; HsT1196; SCC; SCCA-1; SCCA-PD; SCCA1; SSCA1; T4-A; serpin B3

Immunogen: Recombinant fusion protein of human SSCA1/SerpinB3(NP_008850.1).

Conjugate: Unconjugated

Modification: Unmodified