Product Description

Stat1 α/β Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91284-50, BS91284-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P42224

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC IHC FC

All Applications: WB:1:1,000
ICC:1:200
IHC:1:200,
FC:1:100-1:200

Background: STAT1 is a member of the Signal Transducers and Activators of Transcription family of transcription factors. STAT1 is involved in upregulating genes due to a signal by either type I, type II, or type III interferons. In response to IFN-γ stimulation, STAT1 forms homodimers or heterodimers with STAT3 that bind to the GAS (Interferon-Gamma-Activated Sequence) promoter element; in response to either IFN-α or IFN-β stimulation, STAT1 forms a heterodimer with STAT2 that can bind the ISRE (Interferon-Stimulated Response Element) promoter element. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Stat1 α/β polyclonal antibody detects endogenous levels of Stat1 α/β protein.

Bioworld Molecular Weight: 87 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Stat-1α/β on different cell lysates using anti-Stat-1α/β antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: NIH/3T3
Lane 3: MCF-7, ICC staining Stat-1α/β in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Stat-1, CANDF7, DKFZp686B04100, ISGF 3, ISGF3, OTTHUMP00000163552, OTTHUMP00000165046, OTTHUMP00000165047, OTTHUMP00000205845, Signal transducer and activator of transcription 1, Signal transducer and activator of transcription 1, 91kDa, Signal transducer and activator of transcription 1-alpha/beta, Stat1, STAT1, STAT91, Transcription factor ISGF-3 components p91/p84,

Immunogen: peptide

Conjugate: Unconjugated

Modification: Unmodified