Product Description

Stat1 (Phospho-Y701) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: AP0246-50, AP0246-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The Stat1 transcription factor is activated in response to a large number of ligands and is essential for responsiveness to IFN-α and IFN-γ. Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding. Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors. In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellμlar stresses. Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, IF, IP

Alternate Names: Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: Synthetic phosphopeptide derived from human Stat1 around the phosphorylation site of Tyrosine 701.

Dilution: WB 1:500~1:1000
IF: 1:100~1:500
IP: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Stat1(Phospho-Y701) polyclonal antibody detects endogenous levels of Stat1 protein only when phosphorylated at Tyr701.

Molecular Weight: ~ 84 kDa

Pathway: ErbB HER Signaling, MAPK Erk in Growth and Differentiation Pathway(，Inhibition of Apoptosis，Signaling Pathways Activating p38 MAP Kinase

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P42224

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only