STAT1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91282-50, BS91282-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q07954(Human) Q91ZX7(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, IP, , FC
All Applications: WB:1:1,000-1:2,000
FC:1:50-1:100
Background: Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: STAT1 polyclonal antibody detects endogenous levels of STAT1 protein.
Bioworld Molecular Weight: 83/87 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of STAT1 on different lysates using anti-STAT1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293
Lane 2: NIH/3T3, Flow cytometric analysis of MCF-7 cells with STAT1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Alternative Name: STAT1, Signal transducer and activator of transcription 1 91kD, CANDF7, DKFZp686B04100, IMD31A, IMD31B, IMD31C, ISGF 3, ISGF-3, OTTHUMP00000163552, OTTHUMP00000165046, OTTHUMP00000165047, OTTHUMP00000205845, Signal transducer and activator of transcription 1 91kDa, Signal transducer and activator of transcription 1, Signal transducer and activator of transcription 1, 91kD, Signal transducer and activator of transcription 1-alpha/beta, STAT 1, Stat1, STAT1, STAT91, Transcription factor ISGF 3 components p91 p84, Transcription factor ISGF-3 components p91/p84,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified