Product Description

STAT3 (Phospho-S727) Rabbit Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66387-50, MB66387-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The Stat3 transcription factor is an important signaling molecμle for many cytokines and growth factor receptors and is required for murine fetal development . Research studies have shown that Stat3 is constitutively activated in a number of human tumors and possesses oncogenic potential and anti-apoptotic activities . Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding . Transcriptional activation seems to be regμlated by phosphorylation at Ser727 through the MAPK or mTOR pathways . Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage . It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain .

Category: Primary Antibody

Reactivity: Human, Rat

Host: Rabbit

Applications: WB, IF/ICC

Alternate Names: APRF, Signal transducer and activator of transcription 3, Acute-phase response factor

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser727 of human STAT3

Dilution: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of STAT3 (pS727) protein.

Molecular Weight: ~ 86 kDa

Pathway: ErbB HER Signaling, MAPK Erk in Growth and Differentiation Pathway(, Jak Stat: IL-6 Receptor Signaling.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P40763

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Research Use Only