Product Description

Stat3 (phospho-Y705) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4181-50, BS4181-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IP

Alternate Names: Signal transducer and activator of transcription 3, Acute-phase response factor, STAT-3, APRF

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human Stat3 around the phosphorylation site of Tyrosine 705.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Stat3 (Y705) polyclonal antibody detects endogenous levels of Stat3 protein only when phosphorylated at Tyr705

Molecular Weight: ~ 88 kDa

Pathway: Immune Cell Signaling, Jak Stat: IL-6 Receptor Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P40763

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only