STAT5b Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91290-50, BS91290-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P51692(Human) P42232(Mouse) P52632(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP,
All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
Background: Signal transducer and activator of transcription 5A (Stat5a) and Stat5b, which share 96% homology, undergo receptor tyrosine kinase or G protein-coupled receptor-dependent phosphorylation in response to cytokines or growth factors, and then form homo- or heterodimers that translocate to the nucleus, where they initiate transcription. Activation of Stat5a via IL-2, IL-3, IL-7/ GM-CSF, erythropoietin, thrombopoietin and growth hormones influences proliferation, differentiation and apoptosis in lymphohematopoietic cells. Phos-phorylation of Stat5a at Ser127/Ser128 and Ser779 are contigent on ErbB-4-mediated activation of Stat5a. Activation of Stat5b via IL-2, IL-4, CSF-1 and growth hormones influences TCR signaling, apoptosis, adult mammary gland development and sexual dimorphism of liver gene expression. Stat5b is the major liver-expressed Stat5 form that has been shown to fuse with the retin-oic acid receptor a gene in acute promyelocytic leukemias (APLL). Stat5a/b null mice have severely impaired lymphoid development and differentiation.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: STAT5b polyclonal antibody detects endogenous levels of STAT5b protein.
Bioworld Molecular Weight: 90 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of STAT5b on different lysates using anti-STAT5b antibody at 1/1,000 dilution. Positive control:
Lane 1: K562
Lane 2: Hela, ICC staining STAT5b in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: STAT5B, Signal transducer and activator of transcription 5B, STA5B, STAT5, Stat5b, Transcription factor STAT5B,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified