STAT6 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91293-50, BS91293-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P42226(Human) P52633(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC/IF, IHC, IP,
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of proteins that have been designated Stats (signal transducers and activators of transcription). The first members of this family to be described include Stat1α p91, Stat1β p84 (a form of p91 that lacks 38 COOH-terminal amino acids) and Stat2 p113. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Stat3, which becomes activated in response to epidermal growth factor (EGF) and interleukin-6 (IL-6), but not interferon-? (IFN-?) or Stat4, is an additional member of this family. It has been suggested that the phosphorylated forms of both Stat3 and Stat4 form homodimers as well as heterodimers with the other members of the Stat family, and that differential activation of different Stat proteins in response to different ligands should help to explain specificity in nuclear signaling from the cell surface. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Other members of the Stat family include Stat5, which has been shown to be activated by prolactin and by IL-3, and Stat6 (also designated IL-4 Stat), which is involved in IL-4-activated signaling pathways.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: STAT6 polyclonal antibody detects endogenous levels of STAT6 protein.
Bioworld Molecular Weight: 94 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of STAT6 on different lysates using anti-STAT6 antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: Raji, ICC staining STAT6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: STAT6, 12S1644, D12S1644, IL 4 STAT, IL-4 Stat, IL4 STAT, Interleukin 4 Induced, Interleukin 4 Induced Transcription Factor IL4 STAT, Signal transducer and activator of transcription 6, Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced, Signal Transducer And Activator Of Transcription 6 Nirs Variant 1, Signal transducer and activator of transcription 6, interleukin 4 induced, STAT 6, STAT interleukin4 induced, STAT, interleukin4 induced, Stat6, STAT6, STAT6B, STAT6C, Transcription factor IL 4 STAT,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified