Product Description

Stathmin 1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9523-50, MB9523-100

Product: Mouse IgG2b, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P16949 Human

Host: Mouse

Reactivity: Human

Applications: WB, ICC IHC FC

All Applications: WB:1:500
ICC:1:100
IHC:1:50-1:200
FC:1:50-1:100

Background: sphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear. Stathmin’s role in regulation of the cell cycle causes it to be an oncoprotein named oncoprotein 18 (op18). Stathmin (aka op18) can cause uncontrolled cell proliferation when mutated and not functioning properly. If stathmin is unable to bind to tubulin, it allows for constant microtubule assembly and therefore constant mitotic spindle assembly. With no regulation of the mitotic spindle, the cell cycle is capable of cycling uncontrollably resulting in the unregulated cell growth characteristic of cancer cells.

Purification and Purity: Protein G purified.

Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Specificity: Stathmin 1 monoclonal antibody detects endogenous levels of Stathmin 1 protein.

Bioworld Molecular Weight: 17 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining Stathmin 1 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Stathmin 1 monoclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc and trade; 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution., Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Stathmin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with EM1801-19 at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Alternative Name: Op18, pp19, STMN1, C1orf215, LAP18, OP18, C1orf215, Lag, LAP 18, LAP18, Leukemia associated phosphoprotein p18, Leukemia-associated phosphoprotein p18, Metablastin, Oncoprotein 18, OP 18, Op18, p18, p19, Phosphoprotein 19, Phosphoprotein p19, pp17, pp19, PR22, Pr22 protein, Prosolin, Protein Pr22, SMN, Stathmin, Stathmin1, STMN 1, Stmn1, STMN1_HUMAN antibody

Immunogen: Synthetic peptide within C-terminus of human Stathmin 1.

Conjugate: Unconjugated

Modification: Unmodified