Product Description

STK19 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67328-50, BS67328-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P49842

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB (1/500 - 1/2000), IF/ICC (1/50 - 1/200)

Background: Serine/threonine-protein kinase that acts as a key regulator of NRAS signaling by mediating phosphorylation of NRAS at 'Ser-89', thereby enhancing NRAS-binding to its downstream effectors.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of STK19 protein.

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of STK19 expression in Jurkat (A), HepG2 (B), mouse liver (C), rat lung (D), rat brain (E) whole cell lysates., Immunofluorescent analysis of STK19 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Alternative Name: G11; RP1; Serine/threonine-protein kinase 19; Protein G11; Protein RP1

Immunogen: Recombinant full length protein of human STK19

Conjugate: Unconjugated

Modification: Unmodified