Product Description

Strep-Tag II Monoclonal Antibody

Sizes: 50μL, 100μL, 200μL

Catalogue Numbers: E-AB-48009-50, E-AB-48009-100, E-AB-48009-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: Strep-Tag II

Target Synonym: Strep Tag II; Strep II

Conjugation: Unconjugated

Host: Mouse

Species Reactivity: All

Application: WB

Isotype: IgG1

Clonality: Monoclonal

Clone No.: 4H10

Background: The Strep-tag II is a synthetic peptide consisting of eight amino acids ( WSHPQFEK ). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant protein. The Strep-tag II peptide was developed as an affinity tool for the purification of corresponding fusion proteins on streptavidin columns. The peptide recognizes the same pocket of streptavidin where the natural ligand is normally bound so that biotin or its chemical derivatives can be used for competitive elution.

Concentration: 4.5mg/mL

Immunogen: Synthetic peptide corresponding to Strep II tag conjugated to keyhole limpet haemocyanin.

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.4

Purification Method: Protein A/G Purified

Dilution: WB: 1:1000-2000

Calculated MW: 45kDa

Observed MW: 45kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only