Product Description

STUB1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91298-50, BS91298-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UNE7(Human) Q9WUD1(Mouse)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IF, IHC, IP, FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
IP:1:10-1:50

Background: CHIP (carboxy terminus of HSP 70-interacting protein), also designated STIP1 homology and U-box containing protein 1, HSPABP2, NY-CO-7, SDCCAG7 and STUB1, is a cytoplasmic E3 ubiquitin ligase that influences protein ubiquitylation. CHIP interacts with Smad1/Smad4 and blocks BMP signaling through the ubiquitin-mediated degradation of Smad proteins. CHIP controls both association of Hsp70/Hsp90 chaperones with ErbB2 and down-regulation of ErbB2 induced by inhibitors of Hsp90. A 1.3-kb transcript is most abundant in striated muscle (heart and skeletal muscle), with lower expression in pancreas and brain.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: STUB1 polyclonal antibody detects endogenous levels of STUB1 protein.

Bioworld Molecular Weight: 35 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of STUB1 on different lysates using anti-STUB1 antibody at 1/2,000 dilution.
Positive control:
Lane 1: 293
Lane 2: MCF-7
Lane 3: SK-Br-3
Lane 4: Mouse kidney, ICC staining STUB1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: STUB1, CHIP, PP1131, Antigen NY CO 7, Antigen NY-CO-7, C terminus of Hsp70-interacting protein, Carboxy terminus of Hsp70 interacting protein, Carboxy terminus of Hsp70-interacting protein, Carboxy terminus of Hsp70p interacting protein, CHIP, CHIP, CLL associated antigen KW 8, CLL-associated antigen KW-8, E3 ubiquitin protein ligase CHIP, E3 ubiquitin-protein ligase CHIP, Heat shock protein A binding protein 2 (c terminal), HSPABP2, NY CO 7, PP1131, SDCCAG7, Serologically defined colon cancer antigen 7, STIP1 homology and U Box containing protein 1, STIP1 homology and U box containing protein 1 E3 ubiquitin protein ligase, STIP1 homology and U box-containing protein 1, STUB 1, STUB1, UBOX 1, UBOX1,

Immunogen: Recombinant protein within human STUB1 aa 1-200.

Conjugate: Unconjugated

Modification: Unmodified