SUB1 Polyclonal Antibody-BS8687

SUB1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8687-50, BS8687-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P53999

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:100 | IP,1:20 - 1:50

Background: General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 17kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using SUB1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunofluorescence analysis of A549 cells using SUB1 antibody., Immunoprecipitation analysis of 150ug extracts of HL60 cells using 3ug SUB1 antibody. Western blot was performed from the immunoprecipitate using SUB1 antibody at a dilution of 1:500.

Alternative Name: SUB1; P15; PC4; p14; SUB1 homolog

Immunogen: Recombinant fusion protein of human SUB1(NP_006704.3).

Conjugate: Unconjugated

Modification: Unmodified