SUFU Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91299-50, BS91299-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9UMX1(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC IHC
All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: Su(fu) (for Suppressor-of-Fused) is a key negative regulator in the vertebrate Hedgehog signaling pathway. Su(fu) interacts with genes encoding proteins in this signal transduction pathway. In Drosophila, Intracellular transduction of the Hedgehog pathway involves the release of a large complex containing Su(fu). Su(fu) inhibits the activity of the transcription factor Gli1 and interacts with Gli2, Gli3 and the serine/threonine kinase Fused. Su(fu) is widely expressed in adult and embryonic tissues with higher expression in tissues patterned by hegdehog signaling. The Su(fu) gene locus maps to a region that is deleted in glioblastomas, prostate cancer, malignant melanoma and endometrial cancer.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: SUFU polyclonal antibody detects endogenous levels of SUFU protein.
Bioworld Molecular Weight: 54 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of SUFU on 293 cell lysate using anti-SUFU antibody at 1/500 dilution., ICC staining SUFU in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: SUFUH, OTTHUMP00000020374, OTTHUMP00000020377, OTTHUMP00000020379, PRO1280, SU FU, SU(F)U, Su(fu), SUFU, SUFU negative regulator of hedgehog signaling, SUFU, SUFUH, SUFUXL, Suppressor of fused homolog (Drosophila), Suppressor of fused homolog,
Immunogen: Recombinant protein with human SUFU aa 1-250.
Conjugate: Unconjugated
Modification: Unmodified