Product Description

SUMO Conjugating Enzyme UBC9 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB12293-50, MB12293-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P63279

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Background: The process of SUMO-1 conjugation is similar to that seen with ubiquitin and other forms of post-translational protein modification. Like ubiquitin, SUMO-1 is conjugated to its target protein by the coordinated action of ubiquitin conjugation enzymes E1, E2 and E3. Ubc9 (or ube2M) is a highly conserved, 158 amino acid protein that acts as a SUMO-1 conjugating enzyme. Ubc9 binds to target proteins through their SUMO-1-CS (consensus sequence) domains and interacts with SUMO via the structurally conserved amino-terminal domain.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 18 kDa; Observed MW: 18 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunocytochemistry analysis of SUMO Conjugating Enzyme UBC9 in Hela using SUMO Conjugating Enzyme UBC9 antibody, and DAPI., Western blot analysis of UBE2I/UBC9 in 3T3, PC-12, MEF lysates using UBE2I/UBC9 antibody., Western blot analysis of UBE2I/UBC9 in Hela, A549, HL-60 lysates using UBE2I/UBC9 antibody, Immunohistochemistry analysis of paraffin-embedded Human tonsil using UBE2I/UBC9 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: UBE2I; UBC9; UBCE9; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; p18

Immunogen: A synthetic peptide of human UBE2I/UBC9

Conjugate: Unconjugated

Modification: Unmodified