SUPT4H1 Polyclonal Antibody
Catalogue Numbers: BS60490-50, BS60490-100
Sizes: 50µl, 100µl
Alternative Name: Transcription elongation factor SPT4; hSPT4; DRB sensitivity-inducing factor 14 kDa subunit; DSIF p14; DRB sensitivity-inducing factor small subunit; DSIF small subunit; SUPT4H1; SPT4H; SUPT4H
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P63272
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: SPT4 (also designated Suppressor of Ty4 and p14) and SPT5 (also designated DSIF p160) are highly conserved proteins from yeast to humans. Nuclear SPT4 and SPT5 are involved in both DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole)-mediated transcriptional inhibition as well as the activation of transcriptional elongation by the HIV-1 protein Tat. SPT4 binds SPT5 to form the DSIF (DRB-sensitivity-inducing factor) complex, which binds RNA polymerase II and directly regulates elongation. However, SPT5 protein in mitotic HeLa cells migrates more slowly on SDS-PAGE than does SPT5 isolated from interphase cells, as a result of enhanced SPT5 phosphorylation. The C-terminal CTR1 domain of SPT5 is the substrate for P-TEFb phosphorylation, which is critical for SPT5 function as a regulator of transcriptional elongation.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: SUPT4H1 polyclonal antibody detects endogenous levels of SUPT4H1 protein.
Bioworld Molecular Weight: ~ 13 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of SUPT4H1 polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: PC12 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human SUPT4H1.
Conjugate: Unconjugated
Modification: Unmodified