Survivin Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91304-50, BS91304-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O15392(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF, IHC, FC
All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: The baculovirus protein p35 inhibits virally-induced apoptosis of invertebrate and mammalian cells and may function to impair the clearing of virally infected cells by the immune system of the host. This is accomplished at least in part by the ability of p35 to block both TNF- and FAS-mediated apoptosis through the inhibition of the ICE family of serine proteases. Two mammalian homologs of baculovirus p35, referred to as inhibitor of apoptosis protein (IAP) 1 and 2, share an amino-terminal baculovirus IAP repeat (BIR) motif and a carboxy-terminal RING finger. Although the c-IAPs do not directly associate with the TNF receptor (TNF-R), they efficiently block TNF-mediated apoptosis through their interaction with the downstream TNF-R effectors, TRAF1 and TRAF2. Additional IAP family members include ILP (for IAP-like protein) and survivin. ILP inhibits activated caspase-3, leading to the resistance of FAS-mediated apoptosis. Survivin (also designated TIAP) is expressed during the G2/M phase of the cell cycle and associates with microtubules of the mitotic spindle. Increased caspase-3 activity is detected when a disruption of survivin-microtubule interactions occurs.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Survivin polyclonal antibody detects endogenous levels of Survivin protein.
Bioworld Molecular Weight: 16 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: ICC staining Survivin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Survivin antibody. Counter stained with hematoxylin.
Alternative Name: BIRC5, API4, IAP4, API4, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, Apoptosis inhibitor4, Baculoviral IAP repeat containing 5, Baculoviral IAP repeat containing protein 5, Baculoviral IAP repeat-containing protein 5, BIRC 5, BIRC5, BIRC5, EPR 1, IAP4, Survivin variant 3 alpha, SVV, TIAP,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified