Product Description

SUV39H2 Polyclonal Antibody

Catalogue Numbers: BS2900-50, BS2900-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q9H5I1

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Histone-lysine N-methyltransferase SUV39H2, Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher-order chromatin organization during spermatogenesis. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: SUV39H2 polyclonal antibody detects endogenous levels of SUV39H2 protein.

Extra Notes: Western blot (WB) analysis of SUV39H2 polyclonal antibody at 1:500 dilution
Lane1:MCF-7 whole cell lysate(40ug)
Lane2:HCT116 whole cell lysate(40ug)
Lane3:EC9706 whole cell lysate(40ug)
Lane4:K562 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 53 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Histone-lysine N-methyltransferase SUV39H2; Histone H3-K9 methyltransferase 2;
H3-K9-HMTase 2; Lysine N-methyltransferase 1B; Suppressor of variegation 3-9 homolog 2; Su(var)3-9 homolog 2; SUV39H2

Immunogen: Synthetic peptide, corresponding to Human SUV39H2.

Conjugate: Unconjugated

Modification: Unmodified