Product Description

Synaptophysin (L128) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1345-50, BS1345-100

Sizes: 50µl, 100µl

Swiss-Prot: P08247

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Synaptic vesicles participate in a cycle of fusion with the plasma membrane and reformation by endocytosis. Synaptic vesicle protein synaptophysin (SYP) is targeted to early endosomes in transfected fibroblasts and in neuroendocrine cells. SYP is an N-glycosylated intergral membrane protein found in neurons and endocrine cells that associates into hexamers to form a large conductance channel. SYP contains four transmembrane domains and may function as a gap juction-like channel. Membrane cholesterol specfically interacts with SYP to play a role in vesicle formation. Synaptobrevin (VAMP) also binds to SYP and the resultant complex is upregulated during neuronal development, but is absent in exocytosis fusion complex. Thus, the synaptophysin-synaptobrevin complex is not essential for exocytosis, but rather provides a pool of synaptobrevin for exocytosis.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Synaptophysin (L128) polyclonal antibody detects endogenous levels of Synaptophysin protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Synaptophysin (L128) pAb at 1:500 dilution
Lane 1: The Brain tissue lysate of Mouse (40ug)
Lane 2: The Brain tissue lysate of Rat (40ug)
Lane 3: U-87MG whole cell lysate (40ug)
Lane 4: Panc1 whole cell lysate (40ug)
Lane 5: HCT116 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Synaptophysin (L128) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 38 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Synaptophysin; Major synaptic vesicle protein p38; SYP

Immunogen: Synthetic peptide, corresponding to amino acids 101-150 of Human Synaptophysin.

Conjugate: Unconjugated

Modification: Unmodified