SYNPO Polyclonal Antibody Sizes: 50 µl, 100 µl Catalogue Numbers: BS71251-50, BS71251-100 Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: Q8N3V7 Host: Rabbit Reactivity: Human, Mouse, Rat Applications: WB All Applications: WB,1:500 - 1:2000 Background: Synaptopodin is an actin-associated protein that may play a role in actin-based cell shape and motility. The name synaptopodin derives from the protein's associations with postsynaptic densities and dendritic spines and with renal podocytes (Mundel et al., 1997 [PubMed 9314539]).[supplied by OMIM, Mar 2008] Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of extracts of various cell lines, using SYNPO antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. Bioworld Molecular Weight: 125kDa Specificity: Polyclonal Antibodies Alternative Name: SYNPO Immunogen: Recombinant fusion protein of human SYNPO(NP_001159680.1). Conjugate: Unconjugated Modification: Unmodified