Product Description

SYT3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS77483-50, BS77483-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9BQG1

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Ca(2+ sensor involved in Ca(2+-dependent exocytosis of secretory vesicles through Ca(2+ and phospholipid binding to the C2 domain. Ca(2+ induces binding of the C2-domains to phospholipid membranes and to assembled SNARE-complexes; both actions contribute to triggering exocytosis (By similarity. Plays a role in dendrite formation by melanocytes.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of various cell lines, using SYT3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 63kDa

Alternative Name: SytIII; SYT3

Immunogen: Recombinant fusion protein of human SYT3(NP_115674.1).

Conjugate: Unconjugated

Modification: Unmodified