Product Description

SYT5 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76250-50, BS76250-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O00445

Host: Rabbit

Reactivity: Mouse

Applications: WB

All Applications: WB,1:200 - 1:2000

Background: Synaptotagmins, such as SYT5, are a family of type III membrane proteins characterized by cytoplasmic repeats related to protein kinase C (see MIM 176960) regulatory (C2) domains, which are thought to bind calcium. Synaptotagmins may act both as negative regulators of vesicle fusion, allowing fusion in the presence of calcium, and as calcium receptors or sensor molecules (summary by Hudson and Birnbaum, 1995 [PubMed 7597049]).[supplied by OMIM, Feb 2011]

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of Mouse kidney, using SYT5 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 43kDa

Alternative Name: SYT5

Immunogen: Recombinant fusion protein of human SYT5(NP_003171.2).

Conjugate: Unconjugated

Modification: Unmodified