Product Description

TAF II p135/p105 (E977) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2239-50, BS2239-100

Sizes: 50µl, 100µl

Swiss-Prot: O00268/Q92750

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: TAF II p105, also called TAF4B, is a celltype specific transcriptional co-activator that is a component of the TFIID complex. Expressed primarily in B cells and ovarian granulosa cells, TAF II p105 can interact with OCBA/POU2AF1 to activate B cell-specific octamerdependent transcription. Additionally, TAF II p105 plays an important role in co-activating the transcription factor NFκB and is essential for activation of anti-apoptotic genes such as TNFAIP3. TAF II p135, also known as TAF4, TAF2C, TAF2C1, TAF4A or TAFII130, is a 1,085 amino acid subunit of TFIID that accelerates transcriptional activation triggered by thyroid hormone (TR) or retinoic acid (RA). Localized to the nucleus, TAF II p135 contains one TAFH domain and is thought to bind to proteins that contain glutamine-rich domains, such as the transcription factor CREB.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: TAF II p135/p105 (E977) polyclonal antibody detects endogenous levels of TAF II p135/p105 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of TAF II p135/p105 (E977) polyclonal antibody at 1:500 dilution
Lane 1: A2780 whole cell lysate (40ug)
Lane 2: The Ovary tissue lysate of Rat (40ug)
Lane 3: SK-OVCAR3 whole cell lysate (40ug)
Lane 4: CT26 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of TAF II p135/p105 (E977) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 135 kDa,~ 105 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Transcription initiation factor TFIID subunit 4; RNA polymerase II TBP-associated factor subunit C; TBP-associated factor 4; Transcription initiation factor TFIID 130 kDa subunit; TAf (II)130; TAFII-130; TAFII130; Transcription initiation factor TFIID 135 kDa subunit; TAf (II)135; TAFII-135; TAFII135; TAF4; TAF2C; TAF2C1; TAF4A; TAFII130; TAFII135; Transcription initiation factor TFIID subunit 4B; Transcription initiation factor TFIID 105 kDa subunit; TAf (II)105; TAFII-105; TAFII105; TAF4B; TAF2C2; TAFII105

Immunogen: Synthetic peptide, corresponding to amino acids 951-1000 of Human TAF II p135.

Conjugate: Unconjugated

Modification: Unmodified