TAP Tag Monoclonal Antibody (Mix) - RA12056

TAP Tag Monoclonal Antibody (Mix)

Size: 50μL, 100μL

Catalogue Numbers: RA12056-50, RA12056-100

Citations, Manuals and MSDS Available upon request.

Background: The TAP (Tandem Affinity Purification) method is an affinity purification method for the isolation of TAP-tagged proteins along with associated proteins. The TAP tag historically consists of a calmodulin binding peptide (CPB), a tobacco etch virus (TEV) protease cleavage site, and Protein A. However, additional tag combinations have been used with the TAP method including the combination of FLAG tags and HA tags. The TAP method permits the identification of proteins interacting with a particular target protein without any prior knowledge about the function, activity, or composition of the interacting proteins. The TAP tag has been especially useful and deployed with Yeast Tap-tagged ORF clones. These clones contain genomic fusions of the TAP construct and are extremely useful for determining natural protein interactions and expression level variations based on physiological changes.

Condition: PBS, pH 7.4, containing 0.5% BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage Instructions: -20°C/1 year

Applications: WB

Species Cross-Reactivity: Species independent

Source: Mouse

Research Use Only