Product Description

TBP Monoclonal Antibody

Product: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: MB66918-50, MB66918-100

Sizes: 50µl, 100µl

Swiss-Prot: P20226

Host: Mouse

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IHC

Background: TATA-binding protein is a ubiquitously expressed nuclear protein that functions at the core of the general transcription factor protein complex TFIID. TFIID, which contains TBP and 13 TBP-associated factors , contributes to the formation of the transcription pre-initiation complex, an assembly of multiple protein complexes that bind to a gene promoter during the initiation of transcription . Once the pre-initiation complex is formed, RNA polymerase II becomes competent for elongation and transcribes the body of a gene. TBP functions in the recruitment of TFIID by binding to the TATA-box sequence found approximately 25 base pairs upstream of the transcription start site of many protein-coding genes. In addition, many transcriptional activator proteins interact with TBP and various TAF proteins to facilitate recruitment of TFIID and formation of the pre-initiation complex.

Product: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of TBP protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of TBP expression in Hela (A), HepG2 (B), NIH3T3 (C) whole cell lysates., Immunohistochemical analysis of TBP staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 38 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: GTF2D1; TF2D; TFIID; TATA-box-binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit

Immunogen: Recombinant fusion protein of human TBP. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified