Product Description

Tbx21 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91322-50, BS91322-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9JKD8(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, FC

All Applications: WB:1:500
FC:1:50-1:100

Background: T helper (Th) lymphocytes differentiate into two unique subsets, Th1 and Th2, which differ both in function and in the cytokines they secrete. Th1 and Th2 cytokines promote the growth and differentiation of their subset, and inhibit the growth and differentiation of the opposing subset. T-bet (T box expressed in T cells) is a Th1-specific T box transcription factor that controls the expres-sion of the Th1 cytokine, IFN-γ. T-bet also converts effector Th2 cells into the opposing Th1 subset. T-bet is selectively expressed in Th1 cells. The level of T-bet expression is increased by signals mediated by the T cell receptor (TCR). IL-12 also induces an increase in the level of T-bet. T-bet was originally isolated from nuclear extracts of resting and PMA/ionomycin-activated AE7 cells. T-bet is expressed in low levels in AE7 cells, and in increased levels in stimulated AE7.

Purification and Purity: Peptide affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: Tbx21 polyclonal antibody detects endogenous levels of Tbx21 protein.

Bioworld Molecular Weight: 58 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of T-bet on mouse marrow tissue lysate using anti-T-bet antibody at 1/500 dilution., Flow cytometric analysis of Jurkat cells with T-bet antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Alternative Name: T-box protein 21, Tbx21, Tbet, Tblym, T bet, T box 21, T box expressed in T cells, T box protein 21, T box transcription factor TBX21, T cell specific T box transcription factor, T cell specific T box transcription factor T bet, T PET, T-box protein 21, T-box transcription factor TBX21, T-cell-specific T-box transcription factor T-bet, TBET, TBLYM, TBX 21, Tbx21, TBX21_HUMAN antibody TPET, Transcription factor TBLYM,

Immunogen: Synthetic peptide within human T-bet aa 480-530.

Conjugate: Unconjugated

Modification: Unmodified