Product Description

TCEAL1 (E123) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3956-50, BS3956-100

Sizes: 50µl, 100µl

Swiss-Prot: Q15170

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: TCEAL1 (transcription elongation factor A (SII) protein 3), also known as p21, SIIR or pp21, is a member of the TFS-II family. Transcription elongation factors of the TFS-II family are responsible for releasing RNA polymerase II (Pol II) from transcriptional arrest. DNA arresting sites can result in locked ternary complexes if elongating RNA polymerases are trapped. Transcription elongation factors function to activate the intrinsic RNA cleavage activity of RNA polymerases. This allows the RNA polymerase to cleave the nascent transcript, thereby forming a new 3’-terminus to resume elongation. TCEAL1 is a 157 amino acid nuclear protein that is ubiquitously expressed. TCEAL1 may exert its effects via protein-protein interactions with other transcriptional regulators rather than via direct binding of DNA.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: TCEAL1 (E123) polyclonal antibody detects endogenous levels of TCEAL1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of TCEAL1 (E123) pAb at 1:500 dilution
Lane 1: A2780 whole cell lysate (20ug)
Lane 2: PC3 whole cell lysate (20ug)
Lane 3: The Ovary tissue lysate of Rat (40ug)
Lane 4: 3T3-L1 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of TCEAL1 (E123) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 18 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: TCEA-like protein 1; TCEAL1; pp21; SIIR;

Immunogen: Synthetic peptide, corresponding to amino acids 90-143 of Human TCEAL1.

Conjugate: Unconjugated

Modification: Unmodified