Product Description

TCF-1 (F26) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1987-50, BS1987-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: T-cell factor-1 (TCF-1) is a DNA-binding transcriptional activator that isessential for lymphoid cell development. The TCF family of transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. Several alternative splice variants of TCF-1 have been identified, including TCF-1A, which share a conserved amino terminus and differ in the carboxy terminal sequences. The Wnt mediated signaling pathway induces cytosolic β-catenin binding to TCF proteins within the nucleus, leading to the enhanced expression of the Wnt target genes. The β-catenin-TCF complexes are negatively regμlated by the adenomatous polyposis coli (APC) tumor suppressor protein, which phosphorylates β-catenin and, in turn, increases the degradation of cytosolic β-catenin and inhibits the transcriptional activity of the TCF proteins.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IP, IF

Alternate Names: Transcription factor 7, TCF-7, T-cell-specific transcription factor 1, T-cell factor 1, TCF-1, TCF7, TCF1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human TCF-1.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:50~1:200
IF: 1:100~1:500

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: TCF-1 (F26) polyclonal antibody detects endogenous levels of TCF-1 protein.

Molecular Weight: ~ 42 kDa

Pathway: Wntβ catenin signaling，

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P36402

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only