Product Description

TCF3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91323-50, BS91323-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P15923(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, FC

All Applications: WB:1:1,000
FC:1:50-1:100

Background: Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development include Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes. Both homo- and heterooligomers of these proteins are able to distinguish very closely related E box proteins and are believed to play important roles in lineage specific gene expression.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Flow cytometric analysis of 293 cells with TCF3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as theSecondary antibody.

Bioworld Molecular Weight: 67 kDa

Specificity: TCF3 polyclonal antibody detects endogenous levels of TCF3 protein.

Alternative Name: bHLHb21, TCF-3, TCF3, AGM8, bHLHb21, Class B basic helix-loop-helix protein 21, E12, E2A, E2A immunoglobulin enhancer binding factors E12/E47, E47, Helix loop helix protein HE47, Immunoglobulin enhancer-binding factor E12/E47, Immunoglobulin transcription factor 1, ITF1, Kappa-E2-binding factor, MGC129647, MGC129648, Negative vitamin D response element binding protein, NOL1-TCF3 fusion, TCF-3, Tcf3, TFE2, transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47), Transcription factor 3, transcription factor 3 variant 3, Transcription factor E2-alpha, Transcription factor ITF-1, VDIR, VDR interacting repressor, vitamin D receptor-interacting repressor,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified