TCP-1θ Polyclonal Antibody
Catalogue Numbers: BS5960-50, BS5960-100
Sizes: 50µl, 100µl
Alternative Name: T-complex protein 1 subunit theta; TCP1θ; TCP-1 θ; TCP1 θ; TCP1-θ; TCP-1-theta; CCT-theta; CCTθ; CCT-θ; Renal carcinoma antigen NY-REN-15; CCT8; C21orf112; CCTQ; KIAA0002
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P50990
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: The protein TCP-1 (t complex polypeptide 1) is a subunit of the heterooligomeric complex CCT (chaperonin containing TCP-1) present in the eukaryotic cytosol. The CCT of eukaryotic cytosol is composed of eight different subunit species, TCP-1 α, β, γ, δ, ε, ζ, η and θ, each encoded by a different gene. Two ζ subunits have been described: TCP-1 ζ (also designated TCP-1 ζ1) and TCP-1 ζ2. TCP-1 subunits are proposed to have independent functions in folding its in vivo substrates, the actins and tubulins. TCP-1 was first identified in the mouse as relevant for tail-less and embryonic lethal phenotypes. Sequences homologous to TCP-1 have been isolated in several other species, and the yeast TCP-1 has been shown to encode a molecular chaperone for Actin and Tubulin. TCP-1 found in mammalian cells and yeast plays an important role in the folding of cytosolic proteins.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: TCP-1θ polyclonal antibody detects endogenous levels of TCP-1θ protein.
Bioworld Molecular Weight: ~ 59 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of TCP-1θ polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 cell lysate
Lane 2: NIH-3T3 cell lysate
Lane 3: PC12 cell lysate
Immunogen: Synthetic peptide, corresponding to amino acids 404-449 of Human TCP-1θ.
Conjugate: Unconjugated
Modification: Unmodified