Product Description

TDG (K90) Polyclonal Antibody

Catalogue Numbers: BS2053-50, BS2053-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13569

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. G/T mismatch-specific Thymine DNA Glycosylase (TDG) is a nuclear protein which corrects G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymine. TDG also corrects a subset of G/U mispairs inefficiently removed by the more abundant uracil glycosylases. Retinoic acid receptors interact physically and functionally with TDG, enhancing the ability of the retinoid X receptor and the retinoid X receptor/retinoid acid receptor complex to bind to their response elements. TDG interacts with, and is covalently modified by, the ubiquitin-like proteins SUMO-1 and SUMO-2/3, resulting in a reduction of the DNA substrate and AP site binding affinity of TDG. This sumoylation is associated with a significant increase in enzymatic turnover in reactions with a G/U substrate and the loss of G/T processing activity.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: TDG (K90) polyclonal antibody detects endogenous levels of TDG protein.

Extra Notes: Western blot (WB) analysis of TDG (K90) pAb at 1:1000 dilution
Lane1:CT-26 whole cell lysate(40ug)
Lane2:PC12 whole cell lysate(40ug)
Lane3:HCT116 whole cell lysate(40ug)
Lane4:HEK293T whole cell lysate(40ug)
Lane5:Hela whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 46 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: G/T mismatch-specific thymine DNA glycosylase; Thymine-DNA glycosylase; Short name=hTDG; Thymine DNA glycosylase; hTDG

Immunogen: Synthetic peptide, corresponding to amino acids 28-109 of Human TDG.

Conjugate: Unconjugated

Modification: Unmodified