TDP-43/TARDB Polyclonal Antibody-BS6221

TDP-43/TARDB Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS6221-50, BS6221-100

Swiss-Prot: Q13148

Host: Rabbit

TDP-43/TARDB Polyclonal Antibody-BS6221

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IP, , RIP,

All Applications: WB,1:500 - 1:2000 | IP,1:50 - 1:100 | RIP,1:20 - 1:50

Background: HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 42kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using TDP-43/TARDB antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST., Immunoprecipitation analysis of 200ug extracts of SW620 cells using 3ug TDP-43/TARDB antibody. Western blot was performed from the immunoprecipitate using TDP-43/TARDB antibody at a dilution of 1:500.

Alternative Name: ALS10; TDP-43; TARDBP; TDP43

Immunogen: Recombinant fusion protein of human TDP-43/TARDB(NP_031401.1).

Modification: Unmodified

Conjugate: Unconjugated