Product Description

TEC (R220) Polyclonal Antibody

Catalogue Numbers: AP0822-50, AP0822-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P42680

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:1000~1:2000

Background: Tec kinase belongs to a structurally related subfamily of protein tyrosine kinases (PTKs) that includes Btk, Itk (also known as Emt or Tsk), Bmx, and Txk (or Rlk). With the exception of Txk, the members of this subfamily possess a long amino-terminal region consisting of a pleckstrin homology (PH) domain and a Tec homology (TH) domain . Because PH domains bind phosphoinositides with high affinity, the Tec family kinases have been proposed to act downstream of phosphatidylinositol 3-kinase (PI3-kinase) in signaling pathways. Binding of the PH domain with phosphoinositides is probably required for targeting of Tec family kinases to the cell membrane. Tec kinase is activated in response to many upstream signaling events including antigen receptor, RTK, GPCR, and integrin stimulation. Activated Tec kinase directly phosphorylates substrates such as PLC-gamma 2 and BRDG1 docking protein and mediates downstream signaling.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: TEC (R220) polyclonal antibody detects endogenous levels of TEC protein.

Extra Notes: Western blot (WB) analysis of TEC (R220) polyclonal antibody at 1:500 dilution
Lane1:Myla2059 whole cell lysate(40ug)
Lane2:K562 whole cell lysate(40ug)
Lane3:The Spleen tissue lysate of Rat(40ug)
Lane4:The Spleen tissue lysate of Mouse(40ug)

Bioworld Molecular Weight: ~ 58-75 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Tyrosine-protein kinase Tec; TEC; PSCTK4

Immunogen: Synthetic peptide, corresponding to amino acids 190-250 of Human TEC.

Conjugate: Unconjugated

Modification: Unmodified