Product Description

TEL (N405) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1990-50, BS1990-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P41212

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC, IF

All Applications: IHC: 1:50~1:200
IF: 1:50~1:200

Background: Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. Members of the Ets gene family exhibit varied patterns of tissue expression and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5’-GGAA-3’ element. TEL (for translocation, Ets, leukemia), also designated Et V6, is a member of the Ets family that is involved in specific chromosomal translocations in human leukemia and sarcoma.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: TEL (N405) polyclonal antibody detects endogenous levels of TEL protein.

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of TEL (N405) pAb in paraffin-embedded human lung carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of TEL (N405) pAb in paraffin-embedded human esophagus carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of TEL (N405) pAb in paraffin-embedded human stomach carcinoma tissue at 1:5000. cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Transcription factor Et V6; ETS translocation variant 6; ETS-related protein Tel1; Tel; Et V6; TEL; TEL1

Immunogen: Synthetic peptide, corresponding to amino acids 380-430 of Human TEL.

Conjugate: Unconjugated

Modification: Unmodified