Product Description

Tenascin C Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS77607-50, BS77607-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P24821

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: This gene encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration. [provided by RefSeq, Jul 2011]

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 240KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of U-138MG, using Tenascin C antibody at 1:1205 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunohistochemistry of paraffin-embedded Human colon carcinoma using Tenascin C antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunofluorescence analysis of U-87MG cells using Tenascin C Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining.

Alternative Name: 150-225; DFNA56; GMEM; GP; HXB; JI; TN; TN-C; TNC

Immunogen: Recombinant fusion protein of human Tenascin C(NP_002151.2).

Conjugate: Unconjugated

Modification: Unmodified