Terminal Transferase (TdT)
Sizes: 100μg, 1mg
Catalogue Numbers: BY380012-100, BY380012-1000
Citations, Manuals and MSDS Available upon request.
Description: Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. It catalyzes the repeated addition of deoxyribonucleotides to oligodeoxyribonucleotides and 3' -Oh of single - and double-stranded DNA. TdT requires an oligodeoxynucleotide of at least three bases as a primer. The addition of Co2+ in the reacton makes tailing more efficient.
Form: Liquid
Storage buffer: 100mM KAc (pH6.8), 2mM 2-mercaptoethanol, 0.01%Triton X-100 and 50% glycerol.
Purity: >90% as determined by SDS-PAGE.
Applications: Terminal transferase catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules.
Protein length: Terminal Transferase (TdT) is expressed in E. coli.
Nature: Recombinant
Predicted molecular weight: 45 kDa
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week .Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names: DNA nucleotidylexotransferase, 2.7.7.31, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, TDT, Terminal transferase, DNTT, TDT
Species: Bovine
Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
For research use only.