Product Description

TET3 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-93257-60, E-AB-93257-120, E-AB-93257-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: TET3

Target Synonym: BC037432; B430006D22Rik; D230004J03Rik

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat

Application: WB

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: Q8BG87

Background: Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC into 5-hydroxymethylcytosine (5hmC and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC, and conversion of 5fC to 5-carboxylcytosine (5caC. Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG dinucleotides over CpH (H=A, T, and C, hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG (By similarity.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of mouse TET3

Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000

Calculated MW: 194kDa

Observed MW: 230KDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only