TF2A2 Polyclonal Antibody-BS62276

TF2A2 Polyclonal Antibody

Catalogue Numbers: BS62276-50, BS62276-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P52657

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB:1:500~1:1000

Background: Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: TF2A2 polyclonal antibody detects endogenous levels of TF2A2 protein.

Extra Notes: Western blot (WB) analysis of TF2A2 polyclonal antibody at 1:500 dilution
LaneA:H9C2 whole cell lysate
LaneB:AML-12 whole cell lysate
LaneC:HEK293T whole cell lysate
LaneD:A549 whole cell lysate

Bioworld Molecular Weight: ~ 17 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Transcription initiation factor IIA subunit 2; TFIIA p12 subunit; TFIIA-12; TFIIAS; TFIIA-gamma; GTF2A2; TF2A2

Immunogen: Synthetic peptide, corresponding to Human TF2A2.

Conjugate: Unconjugated

Modification: Unmodified