TFEB Polyclonal Antibody - E-AB-93321

TFEB Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-93321-60, E-AB-93321-120, E-AB-93321-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: TFEB

Target Synonym: TFEB; ALPHATFEB; BHLHE35; TCFEB

Research Areas: Cancer, Metabolism, Signal Transduction

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat

Application: WB, IHC, IF

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: P34897

Background: Transcription factor that acts as a master regulator of lysosomal biogenesis, autophagy, lysosomal exocytosis, lipid catabolism, energy metabolism and immune response. Specifically recognizes and binds E-box sequences (5'-CANNTG-3'; efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFEB phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFEB dephosphorylation, resulting in nuclear localization and transcription factor activity.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human TFEB

Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50-1:200

Calculated MW: 53kDa/54kDa/55kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only