TFG Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS91332-50, BS91332-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q92734(Human) Unigene:425970(Mouse) Unigene:24822(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP,
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: Oncogenic rearrangements of the NTRK1 gene, which encodes the Trk A protein, are frequently detected in thyroid carcinomas. Such rearrangements fuse the NTRK1 tyrosine kinase domain to 5'-end sequences of different genes. TRK-T3 contains 1,412 nucleotides of NTRK1 preceded by 598 nucleotides belonging to TFG (TRK-fused gene), a ubiquitously expressed gene located on chromosome 3. The TRK-T3 protein within the TFG region contains a coiled-coil motif that gives the oncoprotein the capability to form complexes. The cytoplasmic TRK-T3 protein binds to and phosphorylates the Shc and SNT1/FRS2 adaptor proteins, both of which are involved in coupling the receptor tyrosine kinase to the mitogen-activated protein kinase pathway by recruiting Grb2/SOS. SHP-1 also interacts with and down-regulates TRK-T3.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: TFG polyclonal antibody detects endogenous levels of TFG protein.
Bioworld Molecular Weight: 55 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of TRK fused gene on different lysates using anti-TRK fused gene antibody at 1/5,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: Hela, ICC staining TRK fused gene in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: TFG, FLJ36137, HMSNP, OTTHUMP00000214045, OTTHUMP00000214046, OTTHUMP00000214047, OTTHUMP00000214048, Protein TFG, SPG57, TF6, TFG, TFG, TRK fused, TRK fused gene, TRK fused gene protein, TRK-fused gene protein, TRKT3, TRKT3 oncogene,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified